Pranitha was born into a Kannadiga family. Her father Subhash is a physician and her mother Jayashri is a Gynaecologist. She made her acting debut in films with the 2010 Kannada film Porki, a remake of the Telugu blockbuster film PokiriAfter the success of Porki, she refused several offers from Kannada films and became choosy about her projects before signing the Telugu film Baava, a love story, she was praised unanimously for her performance. She made her debut in Tamil film, Udhayan. She was then signed up for her second Tamil project Saguni , her biggest release, a film that released in a record 1,150 theaters all over the world. She then appeared in Jarasandha and Bheema Theeradalli, massive success in Karnataka. She then acted in the Kannada film Whistle. She appeared in the Telugu language film Attarintiki Daredi which released in September 2013 and went on to become the Highest grossing Telugu language film of all time collecting 100 crore. It also holds the record of being the 2nd highest grossing Indian film in the USA. She also did Brahma, Pandavulu Pandavulu Thummeda, both the films fared well. Later, she signed another Tamil film Mass and a Telugu film Dynamite. Her future projects are Chuttalabbai, Brahmostavam,Jaggu Dada.
Pranitha Subhash has endorsed brands like Joyalukkas, SVB Silks Salem, Bombay Jewellery, Sri Lakshmi Jewellery, Pondichery and RS Brothers. She was signed up as the brand ambassador of the team Karnataka Bulldozers in the Celebrity Cricket League in its third season in 2013. In October 2014, Pranitha along with Anu Prabhakar was selected as the ambassador for the Jewels of India - a fashion jewellery exhibition at Bangalore.