Rambha was born to Venkateshwara Rao and Usharani in a Telugu kapu naidu family. Her mother tongue is Telugu. She married Indran Pathmanathan, the founder and chairman of Magickwoods, a kitchen and bathroom manufacturing firm based in Canada, on 8 April 2010, in Tirumala. She gave birth to a daughter, Laanya in Toronto on 13 January 2011 and on 31 March 2015 she delivered another baby girl, Sasha.
Rambha made her debut with Kannada movie in 1993, Server Somanna. The same year she acted in a Malayalam film Sargam opposite Vineeth. She again paired up with Vineeth inChampakulam Thachan. She then entered Bollywood and was paired up with popular heroes. In 1993, she completed the movie Tholi Muddhu opposite Prashanth. Her second Tamil movie Ullathai Allitha, which remains her biggest hit till date, gave her a major breakthrough in her career. She has had huge success in South Indian films from the mid 90s till early 2000s. Rambha started her career as a producer with help of her brother with the movie Three Roses in which Jyothika, Laila and Rambha are the lead characters. Unfortunately, the film failed in the box office despite Rambha acting glamorously. Rambha has acted in several languages and maintained a successful balance in Malayalam, Telugu, Tamil, Hindi and Bengali. Her latest film in Malayalam titled Paayum Puli in 2007 got good reviews. Most of her movies are successful. She has acted in 3 Bengali films and now she has 17 Hindi films. After her marriage she had given up films and has judged a very popular Tamil TV show Maanada Mayilada, and the Telugu dance show Dhee. She is now settled in Toronto with her husband and daughter. She is also the brand ambassador for her husband's firm, which has manufacturing facilities in Canada, India, and China. Her upcoming movie is Prathi Kshanam.