Suhasini was born to actor Charuhasan, the prominent Haasan family. She got married to film director Mani Ratnam in 1988 and the couple has a son Nandan born in 1991. She was a student of cinematography from the Madras Film Institute, Suhasini started her career as a camera assistant to Ashok Kumar and later went on to become an actress. She started her career as a make-up artist for several leading actors at that time. Her films Bandhana directed by Rajendra Singh Babu and Suprabhatha, directed by Dinesh Baboo was a blockbuster, and thus became one of the most bankable stars of Kannada cinema of the time. Muttina Haara (1990) with Vishnuvardhan directed by Rajendra Singh Babu, Benkiyalli Aralida Hoovu (1983) directed by K. Balchander, Hendthig Helthini and Amruthavarshini directed by Dinesh Babu were all run away hits. Her work in Mathad Mathad Mallige with Vishnuvardhan directed by Nagathihalli Chandrashekhar, Eradane Maduve and its sequel Mathondu Maduve directed by Dinesh Baboo. Her combination with Dr.Vishnuvardhan is considered as one of the best and rare combinations of South Indian Cinema and the duo have acted together in many blockbuster films that include Bandhana, Suprabhatha, Muthina Haara, Hendthig Helthini, Himapatha, Mathad Mathad Mallige, School Master etc. She was introduced to Malayalam cinema through Padmarajan's Koodevide (1983), which also featured Mammootty. Her role in the AFI Fest-nominated feature Vanaprastham (1999), which starred Mohanlal, was particularly well received. In 1985 Tamil film Sindhu Bhairavi, directed by K. Balachander. In 1996, Suhasini stepped into direction with Indira. She also wrote the screenplay for the film. The project was produced by G. V. Films. Suhasini and her husband Mani Ratnam are involved in the running of their production company Madras Talkies, with G.Srinivasan. Her upcoming movies are Waiting, Paapu, Ommomme and Galla.