Sadhana Sargam is of Maharashtrian origin. She was trained in Indian classical music by her mother, Mrs. Neelatai Ghanekar at an early age, and then by Pandit Jasraj. Her mother introduced her to a composer by the name of Anil Mohile, which later resulted in her involvement with the children's choir. In 1982, she released her first single for the film "Vidhaata". She has also sung 'Om Zaarare' from the Rajinikant performer Kuselan, along with Daler Mehndi and K. S. Chitra. She sang several songs in Hindi and Oriya for music director Kajol-Saroj. Sadhna has sung 1938 Hindi songs in 1546 films and 1111Tamil songs in 536 films. She has reportedly sung 2500 Bengali songs from 1994-2015. She has sung 3467 songs in Marathi. She has sung 15000 songs in 34 Indian languages. She has won all topmost awards in India.
In 2006, Sargam was among the singers who took part in a classical concert to mark Dinanath Mangeshkar's 100th birthday celebrations and earned praise from Hirudhayanath Mangeshkar for her performances in Hindustani classicals. Sargam's contribution to devotional music is well appreciated by many senior singers like Usha Mangeshkar. Sadhana Sargam is one of the prominent forces along with Pandit Jasraj and Jagjit Singh to keep this form of music alive. She was well received in Tamil Nadu. She is very busy in the Tamil music field. Among the Bollywood singers males or females, Sargam sang the most number of songs in Tamil music field. 132 songs of Sargam was listed as of August 2008.